Table 1 Switching properties of Alexa Fluor 647 with (a.) and without (n.a.) use of 405 nm activation laser.
From: Switching behaviour of dSTORM dyes in glycerol-containing buffer
Fluorophore | Evaluated fluorophores/number of measurements/time [days] | Photon number per switch | Equilibrium on-off duty cycle [\(10^{-4}\)] | Survival fraction after illumination for \(400\,\text {s}\) [\(\%\)] | Mean number of switching cycles |
|---|---|---|---|---|---|
Alexa Fluor 647 (n.a.) | 233/2/2 | \(3{,}314\pm 98\) | \(8.7\pm 1.7\) | \(96\pm 4\) | \(30\pm 5\) |
Alexa Fluor 647 (a.) | 531/5/2 | \(3{,}880\pm 190\) | \(6.0\pm 1.5\) | \(32\pm 15\) | \(13\pm 5\) |
