Table 1 PCR primers sequences and expected amplicon size.
From: Rock inhibition promotes NaV1.5 sodium channel-dependent SW620 colon cancer cell invasiveness
Gene | Protein | Forward primers (5′ → 3′) | Reverse primers (5′ → 3′) | Expected size (bp) |
|---|---|---|---|---|
HPRT1 | Hprt1 | TTGCTGACCTGCTGGATTAC | TATGTCCCCTGTTGACTGGT | 119 |
PPIA | Peptidylprolyl isomerase A (PPIA) Cyclophilin A | ACCGCCGAGGAAAACCGTGTA | TGCTGTCTTTGGGACCTTGTCTGC | 129 |
SCN5A | NaV1.5 | TTCCTGGGGTCCTTCTACCT | TTTCCTTCTCCTCGGTCTCA | 103 |
ROCK1 | ROCK1 | AGCGGTTGGAACACCTGATT | AACCGACCACCAGTCACATT | 94 |
RHOA | RhoA | CGCTTTTGGGTACATGGAGT | GAGCAGCTCTCGTAGCCATT | 80 |
