Table 7 Design matrix of experimental runs for riboflavin production by G.oxydans FBFS97 using central composite design (CCD), representing the riboflavin production as affected by fructose (X1), tryptone (X2), K2HPO4 (X3), and CaCl2 (X4), together with the predicted riboflavin production and the levels of variables.
Run | Variables | Riboflavin production mg/L | ||||
|---|---|---|---|---|---|---|
X1 | X2 | X3 | X4 | Experimental value | Predicted value | |
1 | − 2 | 0 | 0 | 0 | 12.21 | 11.63 |
2 | − 1 | − 1 | 1 | − 1 | 10.66 | 9.43 |
3 | 0 | 0 | 2 | 0 | 1.75 | 4.29 |
4 | 0 | 0 | 0 | 0 | 18 | 16.63 |
5 | 1 | 1 | − 1 | 1 | 11.93 | 12.58 |
6 | 0 | 0 | − 2 | 0 | 9.03 | 7.40 |
7 | 0 | 0 | 0 | − 2 | 19.35 | 18.62 |
8 | 1 | 1 | − 1 | − 1 | 12.03 | 10.80 |
9 | 1 | − 1 | 1 | 1 | 0.83 | − 1.25 |
10 | 1 | − 1 | − 1 | − 1 | 13.14 | 13.06 |
11 | − 1 | 1 | − 1 | − 1 | 10.28 | 11.76 |
12 | − 1 | − 1 | − 1 | 1 | 8.46 | 5.88 |
13 | − 1 | 1 | − 1 | 1 | 10.98 | 12.10 |
14 | − 1 | − 1 | − 1 | − 1 | 8.25 | 10.56 |
15 | 0 | − 2 | 0 | 0 | 1.21 | 2.48 |
16 | − 1 | 1 | 1 | 1 | 23.67 | 23.16 |
17 | 0 | 2 | 0 | 0 | 15.91 | 15.54 |
18 | 0 | 0 | 0 | 0 | 16.9 | 16.63 |
19 | 1 | 1 | 1 | − 1 | 3.72 | 5.72 |
20 | 0 | 0 | 0 | 0 | 18 | 16.63 |
21 | 0 | 0 | 0 | 2 | 17.13 | 18.81 |
22 | 0 | 0 | 0 | 0 | 15.7 | 16.63 |
23 | 0 | 0 | 0 | 0 | 14.4 | 16.63 |
24 | − 1 | − 1 | 1 | 1 | 6.86 | 7.85 |
25 | 1 | − 1 | − 1 | 1 | 9.01 | 9.82 |
26 | 1 | − 1 | 1 | − 1 | 0.29 | − 1.11 |
27 | 1 | 1 | 1 | 1 | 13.13 | 10.59 |
28 | 2 | 0 | 0 | 0 | 0.095 | 1.57 |
29 | 0 | 0 | 0 | 0 | 16.8 | 16.63 |
30 | − 1 | 1 | 1 | − 1 | 20.82 | 19.73 |
Levels | Fructose (g/L) | Tryptone (g/L) | K2HPO4 (g/L) | CaCl2 (g/L) | ||
|---|---|---|---|---|---|---|
− 2 | 25.00 | 2.5 | 1.00 | 0.02 | ||
− 1 | 50.00 | 5.00 | 3.00 | 0.03 | ||
0 | 75.00 | 7.00 | 5.00 | 0.04 | ||
1 | 100.00 | 10.00 | 7.00 | 0.05 | ||
2 | 125.00 | 12.5 | 9.00 | 0.06 | ||
