Figure 6
Inhibition of sulfatide-induced platelet P-selectin surface expression in platelets by Dab2 SBP. (a) Platelets were stimulated with ADP and further incubated with sulfatide-free (Lipo-C) or sulfatide-containing liposomes (Lipo-S) in the absence or presence of the indicated peptides. Samples were then fixed, incubated with PE-labeled CD62P (anti-P-selectin) antibody, and analyzed by flow cytometry. The graph represents the median fluorescence intensity for each treatment (mean ± standard deviation) of three independent experiments. Data is represented as a fold increase in fluorescence over ADP-treated platelets. Statistical analysis was carried out using a t test. (b) Color-coded representative immunofluorescence histogram displaying the presence of platelet surface P-selectin for the treatments indicated in (a). The gray plot in the inset indicates the presence of P-selectin in the surface of unactivated platelets. (c) Comparison of the α-synuclein sphingolipid-binding domain with the sulfatide-binding site of Dab2. Asterisks represent identical residues, whereas residues that share common properties are shown as colons. Per ClustalW criteria, semiconservative substitutions are indicated with dots.
