Figure 3

Cyclic dipeptide induces apoptosis in HT-29 cells. Annexin V-FITC/PI double staining was performed to analyze apoptotic cell populations by flow cytometry. HT-29 cells were treated with different doses (50, 100 and 200 μM) of four DKPs as indicated for 24 h. Percentage of the dead (Q1), late apoptotic/necrotic (Q2), early apoptotic (Q3) and viable (Q4) cells were shown in quadrant plot. CTL Control.