Table 2 Cyclic dipeptides induces apoptosis in HT-29 cells in a dose-dependent manner.

From: Induction of mitochondria-mediated apoptosis and suppression of tumor growth in zebrafish xenograft model by cyclic dipeptides identified from Exiguobacterium acetylicum

Concentration in μM

Q1

EAC Q3

LA/NC Q2

Q1 + Q2

Q2 + Q3

Fold changes

DKP-1: HT-29

 CTL

1.0

1.0

1.0

1.0

1.0

 50

1.5

0.4

2.3

1.7

2.3

 100

3.1

0.9

2.6

3.0

3.6

 200

1.1

2.4

9.9

3.3

11.4

DKP-2: HT-29

 CTL

1.0

1.0

1.0

1.0

1.0

 50

1.5

3.0

2.5

1.7

2.7

 100

3.0

4.5

3.5

3.1

3.9

 200

1.2

10.6

11.2

3.5

11.0

DKP-3: HT-29

 CTL

1.0

1.0

1.0

1.0

1.0

 50

1.7

16.5

10.5

3.3

12.7

 100

1.4

17.4

14.9

3.8

15.9

 200

1.3

22.3

15.6

3.8

18.1

DKP-4: HT-29

 CTL

1.0

1.0

1.0

1.0

1.0

 50

1.5

20.5

8.4

3.0

12.6

 100

1.0

18.4

14.6

3.9

15.9

 200

2.1

10.8

18.0

5.5

15.5

  1. Annexin V-FITC/PI double staining was performed to analyze apoptotic cell populations. HT-29 cells were treated with different doses of four DKPs as an indicated time for 24 h. Data are representative of three independent experiments (n = 3). DKP -1—cyclo (l-Pro-l-Leu), DKP -2—cyclo (l-Pro-l-Val), DKP -3—cyclo (l-Pro-l-Phe), and DKP -4—cyclo (l-Pro-l-Tyr). CTL Control, Dead cells (Q1), EAC—early apoptotic cells (Q3), LA/NC—late apoptotic or/and necrotic cells (Q2), Q1 + Q2— total dead cells, Q2 + Q3—total apoptotic cells.
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