Figure 1

RASL-seq assay development: defining transcriptomic profile for Grn KO and Grn WT primary mouse microglia. (a) Experimental design: RASL-seq gene selection classification. (b) Volcano plot identifying signature genes (FC > 1.5 or < 0.667; p value < 0.05). (c) Heatmap for 11 signature genes differentiating Grn WT and Grn KO primary mouse microglia with unbiased clustering (Ward’s method with Euclidian distance) into two groups after removal of one outlier.