Figure 3 | Scientific Reports

Figure 3

From: Discovery of small molecules that normalize the transcriptome and enhance cysteine cathepsin activity in progranulin-deficient microglia

Figure 3

Cysteine cathepsin activity is partially rescued with addition of transcriptional correctors to Grn KO microglia. (a) Fluorescent images of BMV109 and Hoechst in Grn WT and KO cells (Scale bar at 100 μm). (b) Distribution of signal per cell for Grn WT and KO microglia from (a). (c) Quantification of 4 independent experiments, (mean ± SEM), WT n = 21 wells and KO n = 21 wells, unpaired t-test with Welch’s correction in GraphPad Prism v8.11, ***p value ≤ 0.001. (d) BMV109 signal quantification with increasing concentration of nor-BNI and DB-cAMP, (mean ± SEM); quantification for nor-BNI: 3 independent experiments, n = 3–14 wells for each condition, one-way Kruskal Wallis non parametric ANOVA with Dunn’s multiple comparisons comparing each condition to Grn KO in GraphPad Prism v8.11, *p value ≤ 0.05, ***p value ≤ 0.001; DB-cAMP: 4 independent experiments, n = 3–20 wells for each condition, one-way ANOVA with Dunnett’s multiple comparisons comparing each condition to Grn KO in GraphPad Prism v8.11, ****p value ≤ 0.0001.

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