Figure 1 | Scientific Reports

Figure 1

From: Aligned nanofiber scaffolds improve functionality of cardiomyocytes differentiated from human induced pluripotent stem cell-derived cardiac progenitor cells

Figure 1

Schematic outline of the CPC differentiation protocol and immunofluorescence staining of CPC differentiation in 3D aligned nanofiber plates and 2D plates. (a) Outline of the human iPSC-CPC differentiation protocol: Human iPSC-CPCs were plated in 3D aligned nanofiber or 2D plates with the addition of compounds and DMSO control from day 0 to day 3, followed by culture in assay medium until day 7 or day 14 of differentiation. (b) Representative images of cells treated with 1.1 µM 53AH, 10 µM XAV939, or DMSO control at day 14 of differentiation indicate that human iPSC-CPCs are competent for differentiation to cardiomyocytes (express cardiomyocyte marker cTnT) and cells expressing αSMA. (c) A phase contrast image of aligned nanofiber plate. These images were acquired at × 4 magnification.

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