Figure 2 | Scientific Reports

Figure 2

From: The ubiquitin ligase Cullin-1 associates with chromatin and regulates transcription of specific c-MYC target genes

Figure 2

3xFLAG-Cullin expression and localization in HeLa cells. (A) Cullins were N-terminally tagged with 3xFLAG tag. HeLa cells were transfected individually with each 3xFLAG-Cullin, lysed, normalized by Bradford assay, and probed for 3xFLAG by immunoblot. Loading control is β-actin. Molecular weight is depicted in kDa. Uncropped immunoblots are shown in Suppl. Figure 1. (B) HeLa cells were transfected with each 3xFLAG-Cullin and immunofluorescence microscopy was performed against 3xFLAG at × 100 magnification. CUL1, 2, 3, and 5 are expressed in both the nucleus and cytoplasm, while CUL4A and 7 are largely excluded from the nucleus. CUL4B is exclusively nuclear. Size bar indicates 10 μm. Shown are representative images.

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