Figure 1 | Scientific Reports

Figure 1

From: Acapsular Staphylococcus aureus with a non-functional agr regains capsule expression after passage through the bloodstream in a bacteremia mouse model

Figure 1

(a) Mouse experiment flowchart. Mice were injected by the i.p. route with 1 × 108 CFU of S. aureus HU-14. After 24 h mice were sacrificed and blood obtained by cardiac puncture. Blood was plated quantitatively and after an overnight culture all colonies from a plate were harvested and suspended in PBS, and the suspension adjusted to a density of 1 × 109 per ml for further injection to another group of mice. Suspension aliquots were plated for CFU count and colony immunoblots were performed on the grown TSB plates to detect the emergence of any colony producing CP5. (b) Colony immunoblot of strain HU-14 after three passages through mice. The arrows indicate strains Reynolds CP5, Reynolds CP8 and Reynolds NT. These three control strains were added by impregnation of the blot membrane. (c) Magnification of the photograph shown in (b). The arrow indicate colony #1, later identified as P3.1. (d) P3.1 was confirmed as CP5. Positive control was strain Reynolds CP5 (lower left strike). Negative control was Reynolds NT (lower right strike).

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