Figure 6 | Scientific Reports

Figure 6

From: Acapsular Staphylococcus aureus with a non-functional agr regains capsule expression after passage through the bloodstream in a bacteremia mouse model

Figure 6

(a,b) RAW 264.7 cells were stimulated with the S. aureus HU-14 isolate or its derivative P3.1 (Heat-killed bacteria, 107 CFU/ml) for 48 h. Media alone was used as negative control. (a) Photographs at 20 × magnification. (b) The number of mature osteoclasts was quantified by light microscopy using TRAP staining. TRAP + cells with 3 or more nuclei were considered mature osteoclasts. The mean and standard error of cumulative data from 3 independent experiments (6 wells) is shown. Levels of significance were obtained by ordinary one-way ANOVA (Tukey´s multi comparisons test, Brown-Forsythe post-test). (c) TNF-α production was quantified by ELISA in the culture media. The mean and standard error of cumulative data from 3 independent experiments (6 wells) is shown. Levels of significance were obtained by ordinary one-way ANOVA (Tukey’s multi comparisons test, Brown-Forsythe post-test).

Back to article page