Figure 7

Antibiofilm assay was carried out and visualized by scanning electron micrograph (SEM) images by growing (a) Staphylococcus aureus and (b) Bacillis subtilis undisturbed on the surface of calcium sulphate hemihydrate (CSH)/nanohydroxyapatite (nHAP) based nanocement (NC) and drug loaded NC (drug@NC). The plates were further analysed for crystal violet assay (c) S. aureus and (d) B. subtilis. drug@NC = NC + INH, NC + RFP and NC + RFP + INH. *p < 0.05, **p < 0.01, ***p < 0.001.