Figure 3
From: Role of Armadillo repeat 2 and kinesin-II motor subunit Klp64D for wingless signaling in Drosophila
AR2 inhibits Arm function in Wg signaling. Assay for Wg signaling activity in the cells expressing control HA, Arm, AR2 or Arm plus AR2, respectively. The relative luciferase activity indicates the ratio of firefly and renilla luciferase activities. Culture media from tub-Wg S2R+ cell was added 1 day before cell lysis to induce signaling. Overexpression of Arm increases the relative luciferase activity, whereas overexpression of AR2 in the presence or absence of Arm causes a strong reduction of Wg signaling (** p < 0.001, five independent assays).
