Figure 6

The tail domain is essential for the function of Klp64D. MARCM was used to induce Klp64D or Klp64D^Δtail expression in Klp64D^k1 mutant clones in wing imaginal disc. Clones are marked by GFP in (a–d). (a’–d’) show merges of GFP and DAPI channels. (A–A’) Wild-type clones. (B–B’) Klp64D^k1 mutant clones are small. (c–c’) Klp64D^k1 mutant clones with UAS-Klp64D expression are significantly larger than Klp64D^k1 clones. (d–d’) Klp64D^k1 mutant clones are not rescued by Klp64D^Δtail. (e) Quantification of clone sizes shown in (a–d’). ‘Percent GFP clone area’ indicates the fraction of the GFP-positive area in the entire disc (n = 10). Growth defects of Klp64D^k1 mutant clones are suppressed by wild-type Klp64D but not by Klp64D^Δtail.