Figure 3
Large deletions in edited P. tricornutum metabolic genes captured by Nanopore amplicon sequencing. For each (A–E), the name of the target gene as well as the editing enzyme are indicated. The leftmost plot shows normalized read coverage averaged over a 5-bp window for the edited sample (black dots) and the wild-type sample (orange dots) relative to the position in PCR amplicon. Numbering on the x-axis is relative to the ATG start codon for each gene, with sequence upstream indicated by a minus (−) symbol and sequence downstream indicated by a plus (+) symbol. The green vertical line indicates the Cas9 or TevCas9 cleavage site, while the shaded rectangle indicates the ORF. The middle plot is a density plot of deletions > 50-bp. The rightmost plot shows the length and position of deletions > 50-bp relative to their position in the PCR amplicon, with numbering of the x-axis as in the leftmost panel. Each horizontal line indicates a mapped deletion event. Deletions are ordered from longest to smallest. The green line indicates the Cas9 or TevCas9 cleavage site.
