Figure 5
From: Targeting cPLA2 derived lipid hydroperoxides as a potential intervention for sarcopenia
Loss of innervation induces cPLA2 activity and downstream eicosanoids in skeletal muscle. (a) cPLA2 activity in sham and denervated gastrocnemius muscles from male WT mice (n = 11 in triplicate). Statistical significance determined by two-tailed student's t-test. (b) cPLA2 activity in young (n = 8), old (n = 7), Sod1−/− (n = 5), and SOD1G93A (n = 6) gastrocnemius muscles from female (pink) and male (blue) mice performed in triplicate. Statistical significance determined by ordinary one-way ANOVA with Tukey’s post hoc test. *p < 0.05 for designated comparison. Eicosanoid content in gastrocnemius muscles expressed as logx fold change from young control (n = 6) for (c) sciatic nerve transection (7 days denervation) (n = 4), (d) Old (28 month) wild-type (n = 4), and (e) end-stage SOD1G93A mice (n = 4). All plots represent mean ± standard deviation. Statistical significance determined by two-tailed student’s t-test (*p < 0.05 compared to control) with Benjamini–Hochberg FDR correction (#q < 0.05 compared to control).
