Figure 6
From: Targeting cPLA2 derived lipid hydroperoxides as a potential intervention for sarcopenia
In vivo cPLA22 Inhibition protects against denervation-induced muscle hydroperoxide production and atrophy. (a) Experimental design. (b) Percent atrophy of denervated gastrocnemius relative to sham of the same animal in male untreated (n = 35), corn oil-injected (n = 24), and AACOCF3 injected (n = 23) mice 7 days after sciatic nerve transection. Significance determined by two-tailed student’s t-test (vehicle versus AACOCF3). Untreated group shown as a comparison to vehicle treated. (c) Basal hydroperoxide production rate in sham and denervated gastrocnemius fibers from corn oil-injected (n = 8 permeabilized, n = 9 unpermeabilized), and AACOCF3 injected (n = 9 permeabilized, n = 10 unpermeabilized) mice 7 days after sciatic nerve transection. Significance determined by ordinary two-way ANOVA with Tukey’s post hoc test comparing within standard protocol or within not permeabilized or washed protocol. (d) Representative cross-sectional area (CSA) images using H&E stain and 20 × optical zoom (scale bar 100 μm). (e) Histogram of fiber CSA from corn oil injected (n = 7 sham and n = 8 denervated) and AACOCF3 injected (n = 6 sham and denervated) mouse gastrocnemius muscle. Statistical significance determined by Chi square. ~ 500 fibers were quantified per sample. (f) Quantification of average fiber CSA from corn oil injected (black, n = 7 sham and n = 8 denervated) and AACOCF3 injected (blue, n = 6 sham and denervated) mouse gastrocnemius muscle. Statistical significance determined by ordinary two-way ANOVA with Tukey’s post hoc test. (g) Correlation between normalized gastrocnemius mass and average gastrocnemius fiber CSA from corn oil injected (n = 7 sham and n = 8 denervated) and AACOCF3 injected (n = 6 sham and denervated) mice. Statistical significance determined by linear regression. All untreated, corn oil-injected, and AACOCF3-injected mice were male. (h) Basal hydroperoxide production rate in sham and denervated gastrocnemius fibers from female control (n = 7) or Alox15−/− (n = 6) mice 7 days after sciatic nerve transection. Significance determined by ordinary two-way ANOVA with Tukey’s post hoc test. All plots represent mean ± standard deviation. *p < 0.05 for designated comparison.
