Figure 7
From: Targeting cPLA2 derived lipid hydroperoxides as a potential intervention for sarcopenia
AACOCF3 treatment does not decrease cPLA2 pathway protein content. (a) Representative Western blot images and (b) quantifications in whole muscle homogenate and mitochondrial fraction of sham and denervated gastrocnemius muscles from male mice treated with 7 days of corn oil or AACOCF3 injections (n = 5–6). Ponceau images of each blot were taken, then the blots were cut to probe with individual primary antibodies. n = 2 samples were run per blot. Multiple blots were compared by loading a control sample on each blot and normalizing the probed bands to the loading control and then to the control sample. The blots were processed in parallel. Full blot images are available in Supplemental Fig. 4. Statistical significance determined by two-way ANOVA with Tukey’s post hoc test for each protein. All plots represent mean ± standard deviation. *p < 0.05 for designated comparison.
