Figure 1
From: Sclerostin expression in trabecular bone is downregulated by osteoclasts
Localization of sclerostin-positive osteocytes and osteoclasts in trabecular bone. (A) Immunohistochemical analysis of sclerostin in the femora from C57BL/6 male mice at 12 weeks of age (left panel). The boxed areas in the left panel show the high-power field of cortical and trabecular areas. The center panel shows the cortical areas. The right panel shows the trabecular areas. Sclerostin-positive signals (brown). (B) Sclerostin-positive cells/bone area (N/mm2). (n = 5). (C) Histochemical analysis of Sost-Green in the femora from 12-week-old SostGreen/+ knock-in male mice (left panel). The boxed areas in the left panel shows the high-power field of cortical and trabecular areas. Sost-positive signals were observed (green). Nuclei were stained with Hoechst 33342 (blue). (D) Sost-Green-positive cells/number of nuclei (%). (n = 5). (E) Histochemical analysis of TRAP in the tibiae of C57BL/6 male mice at 12 weeks of age. TRAP-positive cells (red). The ROI are indicated by the areas surrounded by the broken lines. (F) Number of osteoclasts/bone surface (N/mm) (n = 6). (G) Double staining of sclerostin and TRAP in the trabecular area of tibiae from C57BL/6 male mice at 12 weeks of age. Black arrows marked sclerostin-negative osteocytes. **p < 0.01. Scale bar, 50 μm.
