Figure 4
From: Sclerostin expression in trabecular bone is downregulated by osteoclasts
Bone resorption and LIF expression in Rankl+/− mice. (A) ELISA analysis of RANKL in serum from WT and Rankl+/− male mice at 12 weeks of age (n = 5). (B) Histological analysis of toluidine blue staining in the trabecular area of the tibiae from WT mice (left panel) and Rankl+/− mice (right panel). (C) Trabecular bone volume of tibiae from WT mice and Rankl+/− mice (n = 5). (D) Levels of CTX in serum from WT and Rankl+/− mice (n = 5). (E) Histochemical analysis of TRAP in the trabecular area of the tibiae from WT mice (left panel) and Rankl+/− mice (right panel). TRAP (red). (F) Number of osteoclasts/bone surface (N/mm) (n = 5). (G) Osteoclast surface/bone surface (%) (n = 5). (H) Immunohistochemical analysis of LIF in the trabecular area of the femur from WT mice (top left panels) and Rankl+/− mice (top right panels). The lower two panels show double-staining of LIF and TRAP. LIF-positive cells were marked by red arrows. TRAP-positive cells were marked by blue arrows. (I) LIF-positive cells/bone surface (N/mm). (n = 5). **p < 0.01. Scale bar, 50 μm.
