Figure 5
From: Sclerostin expression in trabecular bone is downregulated by osteoclasts
Expression of sclerostin, β-catenin, and ALP in Rankl+/− mice. (A) Immunohistochemical analysis of sclerostin in the trabecular area of the femur from WT mice at 12 weeks of age (left panels) and Rankl+/− mice (right panels). (B) Sclerostin-positive cells/bone area (N/mm2). (n = 5). (C) Measurement of sclerostin in bone marrow aspirates from WT mice and Rankl+/− mice using ELISA (n = 5). (D) The expression of β-catenin in the trabecular area of the femur from WT mice (left panels) and Rankl+/− mice (right panels). (E) β-catenin-positive cells/bone surface (N/mm). (n = 5). (F) Immunohistochemical analysis of ALP in the trabecular area of the femur from WT mice (left panel) and Rankl+/− mice (right panel). (G) ALP activity in serum from WT and Rankl+/− mice (n = 5). (H) Histological analysis of toluidine blue staining in the trabecular area of the tibiae from WT mice (top left panel) and Rankl+/− mice (top right panel). The lower two panels show double labeling of calcein. (I) Bone histomorphometry analysis of mineral apposition rate (MAR) in the tibiae from WT mice (left panel) and Rankl+/− mice (right panel). (n = 5). (J) Bone histomorphometry analysis of bone formation rate (BFR) in the tibiae from WT mice (left panel) and Rankl+/− mice (right panel). (n = 5). **p < 0.01. Scale bar, 50 μm.
