Figure 3
Effect of HBx expression on HIF expression and transcriptional activity in HepG2 cells. HepG2-NTCP cells were transduced with Ad-HBx and 24 h later the cells were probed for HBx and Smc6 expression by western blot, uncropped blots are available in Supplementary Fig. 6. In parallel experiments HepG2-NTCP cells were infected with HBV or a mutated virus lacking HBx (HBVX-) (MOI of 200) in the presence or absence of Ad-HBx and the major viral transcript, pregenomic RNA measured at 6 days post-infection (a). HepG2-NTCP cells were transduced with Ad-HBx or Ad-OVA and HBx and HIF-1α expression assessed at selected times after culturing at either 20% or 1% oxygen, uncropped blots are available in Supplementary Figs. 7 and 8 (b). Data is shown from a single experiment and is representative of three independent experiments where the mean data is presented. Statistical analysis was performed with Kruskal–Wallis test, with multiple comparisons adjusted by Dunn’s correction.
