Figure 4 | Scientific Reports

Figure 4

From: Hypoxic gene expression in chronic hepatitis B virus infected patients is not observed in state-of-the-art in vitro and mouse infection models

Figure 4

Effect of silencing viral transcription in HBV transgenic mice on hypoxia target gene transcripts. HBV transgenic mice (n = 6 per group) were treated with liver directed siRNAs targeting the HBx region (siHBV) which is commonly shared by all viral RNAs or with a control siRNA (siCtrl). Seven days later we assessed the efficacy of siHBV silencing by quantifying: serum HBeAg levels (a), HBV RNAs in the liver and hypoxia target gene (CAIX, VEGFA, GLUT1 and PHD2) RNAs (b). Hypoxia target genes values are expressed as ΔCt values by subtracting the Ct value of the housekeeping gene β-actin from Ct value of the gene of interest. Mann Whitney (a) or 2-way-ANOVA with Bonferroni correction (b) analyses were applied with p < 0.05 deemed as significant.

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