Figure 5

Comparing hypoxia gene signatures in HBV infected hepatocytes and humanized liver chimeric mice. Mock or HBV-infected HepG2-NTCP cells (MOI 200) were harvested after 3 or 9 days, lysed and assessed for HIF-1α, HIF-2α or CAIX expression and the housekeeping gene B-actin by western blotting. As a positive control HepG2-NTCP cells were treated with the HIF PHD inhibitor FG4592 (FG, 30 μM) for 24 h and protein lysates analysed by western blotting, uncropped blots are available in Supplementary Fig. 9 (a). Induction of hypoxic genes (Supplementary Table 2) in transcriptomic data of HBV infected primary human hepatocytes⁵⁵, HBV infected human liver chimeric mice and a CHB cohort (b). Fold change was calculated for each of the 80 genes in HBV infection against the healthy controls, where the dotted line represents a twofold change. For the CHB cohort, fold change was calculated from the raw Affymetrix, differential expression was tested using multiple t-tests and significance determined by (adjusted p value < 0.05).