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Figure 1

From: Early overnutrition sensitizes the growth hormone axis to the impact of diet-induced obesity via sex-divergent mechanisms

Figure 1

Cumulative delta body weight (BW) curves between PND 23 and 120 (A; the insets represent the absolute BW gain between PND 23 and 120), circulating GH levels (B) and pituitary GH mRNA levels (C) from male (left panels) and female rats (right panels) subjected or not to overfeeding during lactation (SL vs. NL) and/or HFD after weaning (HFD vs. CD). mRNA copy numbers were determined by qPCR and adjusted by a Normalization Factor (NF) in each sample obtained from the expression levels of three housekeeping genes (β-actin, Hprt and Cyclophilin A). Data are presented as mean ± standard error of the mean (SEM), n = 8 per experimental group. Statistically significant differences were assessed by two-way ANOVA to analyze the effects of litter size and diet and their interactions. When significant differences were found, the data were further analyzed using Newman-Keuls tests to identify simple effects. *P < .05; **P < .01 effect of HFD; a, P < .05, effect of litter size; b, P < .05 interaction of litter size/HFD.

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