Figure 3 | Scientific Reports

Figure 3

From: Early overnutrition sensitizes the growth hormone axis to the impact of diet-induced obesity via sex-divergent mechanisms

Figure 3

Pituitary GHRH-R, GHS-R, SST2, SST5, ghrelin and Pit-1 mRNA levels in male (left panels) and female rats (right panels) subjected or not to overfeeding during lactation (SL vs. NL) and/or HFD after weaning (HFD vs. CD). mRNA copy numbers were determined by qPCR and adjusted by a Normalization Factor (NF) in each sample obtained from the expression levels of three housekeeping genes (β-actin, Hprt and Cyclophilin A). Data are presented as mean ± standard error of the mean (SEM), n = 8 per experimental group. Statistically significant differences were assessed by two-way ANOVA to analyze the effects of litter size and diet and their interactions. When significant differences were found, the data were further analyzed using Newman-Keuls tests to identify simple effects. *P < .05; **P < .01 effect of HFD; a, P < .05, effect of litter size; b, P < .05 interaction of litter size/HFD.

Back to article page