Figure 7

The Confocal Microscopic validation of the soluble curcumin-vancomycin combination mediated bacterial cell membrane using Sytox green-propidium iodide dual staining. (a) Post treatment micrograph stained with Sytox green. (b) Post treatment micrograph stained with Propidium iodide. (c) Merged overlay of panels (a) and (b). (d) Zoomed in bacterial clusters from panel (c) to show the precision of overlap of Sytox green and propidium iodide. (e) Co-localization map of PI with sytox green. (f) Comparative relative intensity and distance plot of sytox green and PI for co-localization validation (all confocal images were analyzed and processed using ZenBlue imaging software).