Figure 1
From: Autophagy regulates trophoblast invasion by targeting NF-κB activity
Effects of autophagy inhibition on the invasive potential of JEG-3 and HTR-8/SVneo cells upon ATG5 or beclin-1 knockdown. (A,B) Western blot for ATG5 and beclin-1 upon treatment of ATG5 or beclin-1 shRNA in JEG-3 and HTR-8/SVneo cells. (C,D) Flux assay with a lysosomal protease inhibitor mixture, E-64d and pepstatin A, in JEG-3 and HTR-8/SVneo cells. Cells were treated with either the mixture of E-64d and pepstatin A or the vehicle, for 4 h. Three different experiments were performed and representative blots are shown with densitometric analysis for these experiments. The amount of LC3-II normalized to the amount of β-actin is represented by a bar graph (*p < 0.05, **p < 0.01, ***p < 0.001). (E,F) Invasion assays performed with JEG-3 and HTR-8/SVneo cells. Cells invading the Matrigel were stained with hematoxylin/eosin and counted under a microscope. Results of the invasion assay are represented in a bar graph generated with data obtained from three different experiments (n = 3, *p < 0.05; lower panel) and representative pictures from three different assays are shown in the upper panel.
