Figure 3
From: Autophagy regulates trophoblast invasion by targeting NF-κB activity
The expression of p65, phosphorylated Akt, and total Akt in autophagy-deficient JEG-3 and HTR-8/SVneo cells. (A,B) Western blot for p65, a subunit of NF-κB, in JEG-3 and HTR-8/SVneo cells expressing GFP or shRNA against ATG5 or beclin-1. The bar graph in the right panel represents p65 expression levels determined by densitometry, normalized to β-actin expression (n = 3, ***p < 0.001, **p < 0.01). (C,D) Phosphorylated Akt and total Akt were analyzed by Western blot using phospho-specific Akt antibody and anti-Akt antibody which recognizes both phosphorylated and unphosphorylated Akt. The bar graphs in the right panel represents phosphorylated and total Akt expression levels determined by densitometry, normalized to β-actin expression (n = 3, ***p < 0.001, **p < 0.01, *p < 0.05).
