Figure 4
From: Autophagy regulates trophoblast invasion by targeting NF-κB activity
The levels of sFlt-1 and TNF-α in autophagy-deficient JEG-3 and HTR-8/SVneo cells. (A,B) sFlt-1 levels were measured by ELISA in the media obtained from JEG-3 and HTR-8/SVneo stable cells upon treatment of ATG5 or beclin-1 shRNA. Results are shown as the mean ± SEM of triplicate observations from three different experiments (n = 3, *p < 0.05, ***p < 0.001). (C,D) TNF-α levels were measured by ELISA in the media obtained from JEG-3 and HTR-8/SVneo stable cells upon treatment of ATG5 or beclin-1 shRNA in JEG-3 and HTR-8/SVneo cells. Results are shown as the mean ± SEM of triplicate observations from three different experiments (n = 3, *p < 0.05, **p < 0.01).
