Figure 5

Effect of aerobic exercise training type on hindlimb muscle expression of genes associated with fatty acid metabolism in ApoE^−/− mice with LEAD. mRNA expression of genes involved in fatty acid uptake (CD36), transport (FABP3) (A), and fatty acid β-oxidation (CPT1β, HSL, UCP2, LCAD and PPAR-δ) (B) in non-ischemic and ischemic gastrocnemius muscle at end of the study, as measured by quantitative real-time PCR. Results in the exercised groups are expressed as an x-fold change relative to SED mice, set at 1 (n = 6 mice per group). Representative blots and quantitative analysis of CD36, CPT1β, and UCP2 protein expression levels in non-ischemic and ischemic gastrocnemius muscle at the study endpoint, as determined by Western blotting (C). Note that only the bands at the adequate molecular weights were shown here: tubulin (55 kDa), CD36 (88 kDA), CPT1β (88 kDa) and UCP2 (37 kDa). Results in the exercised groups are expressed as percentage of SED, set at 100% (n = 4 mice per group). Data were analyzed using one-way ANOVA with Dunnett’s post-hoc test (quantitative real-time PCR data) or Kruskal–Wallis with Dunn’s post-hoc test (Western blot data): ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 vs. SED.