Figure 6 | Scientific Reports

Figure 6

From: Transcriptomics uncovers substantial variability associated with alterations in manufacturing processes of macrophage cell therapy products

Figure 6

Differences between Mreg and Mreg_UKR exemplified by response to IFN-γ. (a) Volcano plot showing differentially expressed genes between the two cell types. Genes increased by ≥ 16-fold are indicated in red. (b) IDO1 protein as measured by flow cytometry. (c) IDO1 mRNA as measured by RT-qPCR. (d) Expression data obtained by RNA-Seq is concordant with RT-qPCR results for Ido1 mRNA and (e) IL32 mRNA. (f) Secretion levels of IFN-γ-induced IP-10 (CXCL10). Data obtained for different donor derived cells is presented as individual points, with mean ± SD for each cell type indicated where appropriate. For panels (b), (c) and (f) significance was calculated using one way ANOVA for each pair-wise comparison with Tukey’s post hoc test in GraphPad Prism and is indicated *p < 0.05, **p < 0.01, ***p < 0.001. Linear regression analysis for data in (d) and (e) was performed in GraphPad Prism.

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