Figure 2
From: C24:0 and C24:1 sphingolipids in cholesterol-containing, five- and six-component lipid membranes
Confocal microscopy 3D (z-stack) images of fluorescent giant unilamellar vesicles containing 0.4% RhoPE. DOPC:lSM:Chol (2:1:1) + 30% lCer (A), + 30% nCer (B), + 15% lCer & 15% nCer (C); DOPC:nSM:Chol (2:1:1) + 30% lCer (D), + 30% nCer (E), + 15% lCer & 15% nCer (F); DOPC:lSM:nSM:Chol (2:0.5:0.5:1) + 30% lCer (G), + 30% nCer (H), + 15% lCer and 15% nCer (I). Scale bars: 10 μm. Temperature was 23 ± 1 °C.
