Figure 1

The principle and an application example of the Double-Flp-In technique. (a) The integration of two simultaneously transfected plasmids encoding two genes of interest can be achieved into a single Flp recombination target (FRT) site. (b) As a proof of concept, we transfected HEK293 cells with the coding sequences for the human organic cation transporter 1 (OCT1) and cytochrome P450 isoform 2C19 (CYP2C19). Together, these proteins will facilitate the uptake of the anti-malarial prodrug proguanil and the metabolism to its active metabolite cycloguanil. Protein icons of OCT1 and CYP2C19 and chemical structures were created using The PyMOL Molecular Graphics System, Version 1.3, Schrödinger, LLC, www.pymol.org.