Table 2 Kinetic parameters of α-L-fucosidase (Amuc_0010), β-hexosaminidases (Amuc_0369 and Amuc_2136) and β-galactosidases (Amuc_0771 and Amuc_1686) with synthetic substrates and HMOS.
Substrate | Vmax (μM min−1) | kcat (min−1) | KM (μΜ) | kcat/KM (min−1 μM−1) | |
|---|---|---|---|---|---|
a-L-fucosidase | Amuc_0010 | ||||
PNP-Fucose | 19.76 ± 11.30 | 2.27E + 04 | 841.23 ± 46.72 | 27.07 | |
2′-Fucosyllactose | 25.13 ± 6.80 | 152.08 | 1.03 × 104 ± 6.98 × 103 | 0.02 | |
β-hexosaminidases | Amuc_0369 | ||||
PNP-GlcNAc | 55.64 ± 2.02 | 4.02 × 104 | 323.38 ± 43.64 | 124.50 | |
Lacto-N-triose II | 982.61 ± 37.68 | 1.42 × 104 | 3,980 ± 210.30 | 3.56 | |
Amuc_2136 | |||||
PNP-GlcNAc | 109.46 ± 4.22 | 8.93 × 104 | 714.55 ± 47.96 | 125.10 | |
Lacto-N-triose II | 443.75 ± 47.20 | 1.45 × 104 | 2,435.82 ± 289.51 | 5.95 | |
β-galactosidases | Amuc_0771 | ||||
PNP-Galactose | 60.08 ± 7.02 | 825.63 | 2,599 ± 565.27 | 0.34 | |
Lacto-N-tetraose | 302.08 ± 53.71 | 4.42 × 103 | 1,223 ± 171.70 | 3.61 | |
Amuc_1686 | |||||
PNP-Galactose | 29.34 ± 3.80 | 1.63 × 103 | 319.40 ± 259.30 | 4.21 | |
