Figure 2 | Scientific Reports

Figure 2

From: Mito-oncology agent: fermented extract suppresses the Warburg effect, restores oxidative mitochondrial activity, and inhibits in vivo tumor growth

Figure 2

In vitro results of A250 treatment. (A) The efficacy of A250 was screened on different cancer cell lines. The mean IC50 was calculated from at least three measurements (Mean ± SD, one-way ANOVA, *p < 0.05, ***p < 0.001). (B) Mitochondrial-derived apoptosis was validated with an immunoblot assay. The significant increase of cytochrome c in the cytosolic fraction indicates that the mitochondrial outer membrane permeability increased after treatment. β-actin was used as the loading control. (C,D) GO interpretation of the iTRAQ results of the in vitro-treated melanoma cells shows significant upregulation of metabolic and respiratory processes. In contrast, proteins related to RNA metabolic processes and gene expression were downregulated by the treatment with A250. (The unedited pictures of SDS-gel electrophoresis and immunoblot can be found in the Supplementary information. SI—Fig. 1–3).

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