Figure 2
From: Multidrug transporter MRP4/ABCC4 as a key determinant of pancreatic cancer aggressiveness
MRP4 silencing reduces tumor growth and tumorigenicity in PANC1 xenografts. Swiss nude mice were subcutaneously injected either with PANC1-MRP4sh2, PANC1-MRP4sh1 clones or PANC1-scramble as a control. Each group contained 8–11 animals and the data shown represents measurements of one of two experiments. (a; left) Tumors were measured with a caliper three times a week for 32 days post-inoculation and tumor volume was calculated as described in “Materials and Methods”. (a; middle) Representative pictures of the dissected xenografts of each group at the end of the experiment. (a; right) Tumor weight was measured with a scale after dissection. (b) PANC1-scramble, PANC1-MRP4sh2 and PANC1-MRP4sh1 xenografts were processed for histological (H&E staining), MRP4, Ki67, and EGFR immunostaining evaluation. Nuclei were counterstained with hematoxylin. Representative images are shown. Cell number per field, proliferative index, MRP4 and EGFR scores were determined by H&E, Ki67, MRP4, and EGFR staining respectively, and are shown as bar plots (right). Data is shown as mean ± SEM. Statistics: Linear regression and one-way ANOVA followed by Tukey’s t-test. ***p < 0.001 and **p < 0.01.
