Figure 3
Fluctuation of c-Myb level during differentiation of hematopoietic cells. (a) Gating strategy for Lin−ScaI+c-Kit+ hematopoietic stem cells (HSC), Lin−ScaI−c-Kit+ (LSK), and Lin−ScaI−c-Kit− progenitor fractions from the bone marrow mononuclear cell fraction that was negative for interleukin (IL)-7 receptor and lineage-affiliated antigens (Lin−). The LSK fraction was subdivided into FcγRloCD34+, FcγRhiCD34+, and FcγRloCD34− populations, representing common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP), and megakaryocyte/erythroid progenitors (MEP), respectively. FSC, forward scatter; SSC, side scatter. (b) Relative c-Myb expression level in HSC or LSK subsets was compared between WT/WT (n = 5) and TA/TA mice (n = 6). Results are shown as means ± SD. (c) Relative c-Myb expression levels for each population were identified by histogram analysis.
