Figure 4

Diminished decrease in c-Myb according to differentiation of erythroid progenitor and attenuation of its development in TA/TA mice. (a) In Lin⁻ cells, LSK and HSC subsets were detected with anti-ScaI and c-Kit antibodies, and CD71^loCD11b^lo and CD71⁺ subsets were identified using anti-CD71 and CD11b antibodies simultaneously. (b) Relative expression levels of c-Myb in CD71^loCD11b^lo and CD71⁺ subsets from Lin⁻ cells in WT/WT mice (n = 6) or TA/TA mice (n = 5) were determined. Results are shown as means ± SD. P-values were estimated using Student’s t-test. (c) The Lin⁻CD71⁺ subset was further divided into three subgroups (c-Myb^lo, c-Myb^med, and c-Myb^hi) according to c-Myb expression. Representative histograms of WT/WT and TA/TA mice were shown. (d) Existence of the three subgroups in the Lin^-CD71⁺ subset derived from histogram data (c) and compared between WT/WT (n = 6) and TA/TA mice (n = 5). P-values were estimated using Student’s t-test. (e) The proportions of CD71⁺ fraction in Lin⁻ cells, which consists of three subgroups with different c-Myb expression levels, were compared between WT/WT mice (n = 6) and TA/TA mice (n = 5). P-values were estimated using Student’s t-test. (f) The correlation between c-Myb expression and c-Kit in Lin⁻CD71⁺ subset was ascertained. (g) The schema proposed in this study is shown, indicating fluctuations in c-Myb and 3 lineage markers (c-Kit, CD71, and ScaI) during differentiation of the Lin⁻ erythroid lineage of wild-type mice. The broken line indicates the stage at which progression is disturbed in the Lin⁻ erythroid lineage of TA/TA mice.