Figure 4
KDM4A overexpression reverses the growth inhibitory effects of RFX5 silencing in HCC. (A,B) Clonogenicity assay of RFX5 sgRNAs (RFsg1, RFsg3)-transfected HepG2 cells and rescued with retroviral vector or retroviral expressing KDM4A vector (A), and clone number quantification derived from triplicate experiments (B). (C,D) Clonogenicity assay of RFX5 sgRNAs (RFsg1, RFsg3)-transfected MHCC-97H cells and rescued with retroviral Consg vector or retroviral expressing KDM4A vector (C), and the quantification of clone numbers from three independent experiments (D). (E,F) MHCC-97H cells, which were modified using lentivirus expressing espCas9 and sgRNAs against non-targeting control (Consg) or RFX5 (RFsg1) alone, and then transduced with retroviral KDM4A, were subcutaneously implanted in BALB/c Nude mice (n = 4 per group). Tumor volumes were determined via calipers (E). Extraction and weighing of tumors were done 60 days after MHCC-97H implantation (F). *P < 0.05, **P < 0.01, ***P < 0.001.
