Figure 7
RFX5-KDM4A negatively regulates p53 and downstream pathway. (A,B) Total proteins from MHCC-97H (A) and HepG2 (B) cells transduced with either KDM4A, FLAG-RFX5 or lentiviral vector control were determined with Western blot. (C,D) Total proteins from MHCC-97H (C) and HepG2 (D) cells transduced with lentiviral sgRNA targeting RFX5 together with either retroviral control vector or KDM4A were determined with Western blot. (E) Total proteins from xenograft tumors were determined with Western blot analysis. (F) The mechanism underlying RFX5 and KDM4A in HCC progression.
