Figure 1

RT-qPCR quantification of abundance of mRNA transcripts of Ddx3y (a) or Eif2s3y (b). The experiments were performed using extracts from either cardiac left ventricles, skeletal muscle, liver, spleen or primary lung fibroblasts from either WT or UtyGT mice. Relative expression values corresponded to the ratios of Delta Ct values obtained for the gene of interest vs. those obtained for the Rps16 normalizing gene.