Figure. 1

Moderate SMFs enhance CD8⁺ T cell granule and cytokine secretion at 72 h stimulation. (A) Cytokine/granule production of stimulated mouse CD8⁺ T cells analyzed by flow cytometry. Cell samples were stimulated with anti-CD3 and anti-CD28 antibodies in the presence of 0.3 T or 0.6 T permanent magnets, and control cells were treated without magnets. Cell samples with no stimulation were used to show the baseline of cytokine secretion. (B) Percentage statistics for the expression of GzmB, IFNγ and TNFα of CD8⁺ T cells stimulated for 72 h (B, n = 10). (C) Relative transcriptional levels of Gmzb, Tnfa, and Ifng in 0.3 T SMF-treated and control CD8⁺ T cells (n = 6). The cell samples were stimulated with anti-CD3 and anti-CD28 antibodies for 72 h. All the relative transcription levels of target genes were normalized to β-actin. Data were analyzed by Student’s t-test; NS, no significance, *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. Error bars indicate the s.e.m. Data are representative of or combined from at least three independent experiments.