Figure. 3

Moderate SMFs improves ATP production and mitochondrial respiration of CD8⁺ T cells. (A) The relative intracellular ATP concentration was measured in CD8⁺ T cells stimulated with anti-CD3 and anti-CD28 antibodies for 72 h (n = 5). (B) OCR of stimulated CD8⁺ T cells at baseline and in response to oligomycin, FCCP, and rotenone with antimycin as detected by the Seahorse MitoStress assay. (C) Baseline OCR of stimulated CD8⁺ T cells (n = 4). (D) ATP-linked OCR (baseline OCR minus the OCR in the presence of oligomycin) of stimulated CD8⁺ T cells (n = 4). (E) The spare respiratory capacity (SRC) of stimulated CD8⁺ T cells (n = 4). (F) ECAR of stimulated CD8⁺ T cells at baseline and in response to glucose, oligomycin, and 2-DG as detected by the Seahorse MitoStress assay. (G) Baseline ECAR of stimulated CD8⁺ T cells (n = 4). (H) ATP concentration of knockdown CD8⁺ T cells compared with that in cells transfected with vectors in the presence or absence of magnets (n = 5). Cell samples were treated with 0.3 T magnets, and samples treated without magnets were used as controls. Data were analyzed by Student’s t-test; NS, no significance, *p < 0.05; **p < 0.01; ***p < 0.001. Error bars indicate the s.e.m. Data are representative of or combined from at least three independent experiments.