Figure. 4
Candidate magnetoreceptor genes involve in modulating the transcription of Uqcrb and Ndufs6. (A) Relative transcriptional levels of candidate magnetoreceptor genes Isca1, Cry1 and Cry2 in 0.3 T SMF-treated stimulated CD8+ T cells and control cells (n = 4–6). (B) Analysis of mRNA levels of Isca1, Cry1 and Cry2 in control and knockdown CD8+ T cells (n = 4–5). (C and D) Analysis of Uqcrb and Ndufs6 mRNA levels in control and Isca1 or Cry1/Cry2 knockdown CD8+ T cells treated without magnets (C) or with 0.3 T magnets (D) (n = 5). All the relative transcription levels of target genes were normalized to β-actin. (E–G) Cytokine/granule production of knockdown CD8+ T cells cultured in the presence or absence of 0.3 T magnets analyzed by flow cytometry. Cell samples were stimulated with anti-CD3 and anti-CD28 antibodies in the presence of 0.3 T magnets, and control cells were treated without magnets. Cell samples with no stimulation were used to show the baseline of cytokine secretion. (H–M) Percentage (H–J) and MFI (K–M) statistics for the expression of GzmB, IFNγ and TNFα of knockdown CD8+ T cells (n = 5–6). Cells transfected with shRNA-Isca1 or shRNA-Cry1/Cry2 were compared with Vector. (N) ATP concentration of knockdown CD8+ T cells compared with that in cells transfected with vectors in the presence or absence of magnets (n = 4). Data were analyzed by Student’s t-test; NS, no significance, *p < 0.05; **p < 0.01; ***p < 0.001. Error bars indicate the s.e.m. Data are representative of or combined from at least three independent experiments.
