Figure 6

Moderate SMFs promote the antitumor response of CD8⁺ T cells in vivo. PyMT mice were exposed to 0.6 T magnetic plates, and littermates exposed to resin fiber plates without magnets were used as controls. Tumor onset (A; n = 30) and tumor growth (B; n = 17) of PyMT mice were constantly monitored. (C) Hematoxylin and eosin-stained mammary tumor sections from PyMT mice (magnification, × 40 and × 400; scale bars, 200 mm). (D) Percentage statistics for CD4⁺ T cells, CD8⁺ T cells, and the CD8⁺/CD4⁺ T cell ratio among tumor-infiltrating T cells in PyMT mice as analyzed by flow cytometry (n = 6). (E) Percentage statistics for the expression of CD69, CD44 and CD25 in tumor-infiltrating CD8⁺ T cells in PyMT mice as analyzed by flow cytometry (n = 5). (F) Cytokine/granule production of tumor-infiltrating CD8⁺ T cells in PyMT mice as analyzed by flow cytometry. (G, H) Percentage (G) and MFI (H) statistics for the expression of GzmB (n = 5), IFNγ (n = 6) and TNFα (n = 6) in tumor-infiltrating CD8⁺ T cells in PyMT mice as analyzed by flow cytometry. Data were analyzed by log-rank test (A), two-way ANOVA (B), or Student’s t-test (D, E, G) (NS, no significance, **p < 0.01; ***p < 0.001, ****p < 0.0001). Error bars indicate the s.e.m.