Figure 1

Substrate microtopography properties and cell adhesion with different coatings. a AFM 3D renderings and surface profiles of the inscribed topographies (from left to right: 0.5 μm, 1.0 μm and 2.8 μm). b Immunolabelling of MDCK cells on different coatings at different cell seeding time points. Actin (phalloidin stain, green) was labelled together with pFAK (red) and chromatin (DAPI stain, blue). The sketch of the topography illustrates the presence of a microtopography. Scale bars are 10 μm.