Figure 2

MDCK II cell colony growth and migration on different topographies. a Phase contrast images of MDCK cells during colony growth on surfaces with different topographic features. The images were taken 1 h (top) and 24 h (bottom) after seeding. Scale bars are 100 μm. The sketch illustrates the division of the field to microtopographic and flat regions. b–e, Progression of orientation, coherency, mean flow magnitude, and order parameter, during cell colony growth and migration.