Figure 3

Analysis of the organization of focal adhesions and actin cytoskeleton. a The analysis on the focal adhesion organization on the substrates was performed by using fast Fourier transform (FFT) of the vinculin image and the brightfield image of the underneath topography. Scale bars 5μm. b Quantification of the focal adhesion orientation and periodicity was performed by measuring and comparing the length and the angle formed with the horizontal axis of the first order diffraction signal of the two channels (see text for the related equations (3) and (4)). c Actin stress fibres at the basal plane as defined by phalloidin staining. Scale bars 5μm.